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Comparative Tissue Distribution Study of Multiple Bioactive Components of Xiao-Yao Powder in Young and Old Rats

Received: 14 August 2022     Accepted: 26 August 2022     Published: 19 September 2022
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Abstract

Xiao-Yao Powder (XYP) has been used for the treatment of liver function disorder, depression and comprehensive syndromes of perimenopausal women for over 2000 years. The present work aims to reveal the tissue distribution characteristics of XYP in young and old female rats contrastively to evaluate rationality and compatibility of herbal medicine, in terms of efficacy and toxicity. An ultra-performance liquid chromatography–tandem mass spectrometry method was used to detect the 14 representative components (albiflorin, paeoniflorin, ferulic acid, senkyunolide I, quercetin, isoliquiritigenin, atractylenolide III, ligustilide, atractylenolide II, liquiritin, liquiritigenin, saikosaponin c, glycyrrhizic acid, and saikosaponin a) in both 2-month-old and 18-month-old female rats. After oral administration of XYP extract (4 g/kg), rats’ tissues (heart, liver, spleen, lung, kidney, brain, stomach, uterus, ovary and small intestine) were taken at time points immediately. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped in positive and negative ionization modes by multiple reaction monitoring (MRM) and an electrospray ionization source. All validation parameters met the acceptance criteria according to regulatory guidelines. The tissue distribution study of XYP confirmed that 14 analytes from the two age groups were mainly distributed in ovary, uterus and liver, which is consistent with the characteristics of XYP in treating liver depression and perimenopausal syndrome. Compared with the young rats, the tissue concentrations of most analytes in old rats group decreased, with the exception of senkyunolide I and Atractylenolide III showing higher levels in brain. The identified target tissues of XYP may help in understanding its pharmacological action in vivo.

Published in Science Journal of Analytical Chemistry (Volume 10, Issue 3)
DOI 10.11648/j.sjac.20221003.13
Page(s) 49-67
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Xiao-Yao Powder (XYP), UPLC-MS/MS, Tissue Distribution

References
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    Jinlian Gu, Mingyue Xu, Fan Zeng, Yue Na, Xinyu Zhong, et al. (2022). Comparative Tissue Distribution Study of Multiple Bioactive Components of Xiao-Yao Powder in Young and Old Rats. Science Journal of Analytical Chemistry, 10(3), 49-67. https://doi.org/10.11648/j.sjac.20221003.13

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    Jinlian Gu; Mingyue Xu; Fan Zeng; Yue Na; Xinyu Zhong, et al. Comparative Tissue Distribution Study of Multiple Bioactive Components of Xiao-Yao Powder in Young and Old Rats. Sci. J. Anal. Chem. 2022, 10(3), 49-67. doi: 10.11648/j.sjac.20221003.13

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    AMA Style

    Jinlian Gu, Mingyue Xu, Fan Zeng, Yue Na, Xinyu Zhong, et al. Comparative Tissue Distribution Study of Multiple Bioactive Components of Xiao-Yao Powder in Young and Old Rats. Sci J Anal Chem. 2022;10(3):49-67. doi: 10.11648/j.sjac.20221003.13

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  • @article{10.11648/j.sjac.20221003.13,
      author = {Jinlian Gu and Mingyue Xu and Fan Zeng and Yue Na and Xinyu Zhong and Lu Zhang and Fang Geng and Ning Zhang},
      title = {Comparative Tissue Distribution Study of Multiple Bioactive Components of Xiao-Yao Powder in Young and Old Rats},
      journal = {Science Journal of Analytical Chemistry},
      volume = {10},
      number = {3},
      pages = {49-67},
      doi = {10.11648/j.sjac.20221003.13},
      url = {https://doi.org/10.11648/j.sjac.20221003.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20221003.13},
      abstract = {Xiao-Yao Powder (XYP) has been used for the treatment of liver function disorder, depression and comprehensive syndromes of perimenopausal women for over 2000 years. The present work aims to reveal the tissue distribution characteristics of XYP in young and old female rats contrastively to evaluate rationality and compatibility of herbal medicine, in terms of efficacy and toxicity. An ultra-performance liquid chromatography–tandem mass spectrometry method was used to detect the 14 representative components (albiflorin, paeoniflorin, ferulic acid, senkyunolide I, quercetin, isoliquiritigenin, atractylenolide III, ligustilide, atractylenolide II, liquiritin, liquiritigenin, saikosaponin c, glycyrrhizic acid, and saikosaponin a) in both 2-month-old and 18-month-old female rats. After oral administration of XYP extract (4 g/kg), rats’ tissues (heart, liver, spleen, lung, kidney, brain, stomach, uterus, ovary and small intestine) were taken at time points immediately. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped in positive and negative ionization modes by multiple reaction monitoring (MRM) and an electrospray ionization source. All validation parameters met the acceptance criteria according to regulatory guidelines. The tissue distribution study of XYP confirmed that 14 analytes from the two age groups were mainly distributed in ovary, uterus and liver, which is consistent with the characteristics of XYP in treating liver depression and perimenopausal syndrome. Compared with the young rats, the tissue concentrations of most analytes in old rats group decreased, with the exception of senkyunolide I and Atractylenolide III showing higher levels in brain. The identified target tissues of XYP may help in understanding its pharmacological action in vivo.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Comparative Tissue Distribution Study of Multiple Bioactive Components of Xiao-Yao Powder in Young and Old Rats
    AU  - Jinlian Gu
    AU  - Mingyue Xu
    AU  - Fan Zeng
    AU  - Yue Na
    AU  - Xinyu Zhong
    AU  - Lu Zhang
    AU  - Fang Geng
    AU  - Ning Zhang
    Y1  - 2022/09/19
    PY  - 2022
    N1  - https://doi.org/10.11648/j.sjac.20221003.13
    DO  - 10.11648/j.sjac.20221003.13
    T2  - Science Journal of Analytical Chemistry
    JF  - Science Journal of Analytical Chemistry
    JO  - Science Journal of Analytical Chemistry
    SP  - 49
    EP  - 67
    PB  - Science Publishing Group
    SN  - 2376-8053
    UR  - https://doi.org/10.11648/j.sjac.20221003.13
    AB  - Xiao-Yao Powder (XYP) has been used for the treatment of liver function disorder, depression and comprehensive syndromes of perimenopausal women for over 2000 years. The present work aims to reveal the tissue distribution characteristics of XYP in young and old female rats contrastively to evaluate rationality and compatibility of herbal medicine, in terms of efficacy and toxicity. An ultra-performance liquid chromatography–tandem mass spectrometry method was used to detect the 14 representative components (albiflorin, paeoniflorin, ferulic acid, senkyunolide I, quercetin, isoliquiritigenin, atractylenolide III, ligustilide, atractylenolide II, liquiritin, liquiritigenin, saikosaponin c, glycyrrhizic acid, and saikosaponin a) in both 2-month-old and 18-month-old female rats. After oral administration of XYP extract (4 g/kg), rats’ tissues (heart, liver, spleen, lung, kidney, brain, stomach, uterus, ovary and small intestine) were taken at time points immediately. The detection was performed on a triple-quadrupole tandem mass spectrometer equipped in positive and negative ionization modes by multiple reaction monitoring (MRM) and an electrospray ionization source. All validation parameters met the acceptance criteria according to regulatory guidelines. The tissue distribution study of XYP confirmed that 14 analytes from the two age groups were mainly distributed in ovary, uterus and liver, which is consistent with the characteristics of XYP in treating liver depression and perimenopausal syndrome. Compared with the young rats, the tissue concentrations of most analytes in old rats group decreased, with the exception of senkyunolide I and Atractylenolide III showing higher levels in brain. The identified target tissues of XYP may help in understanding its pharmacological action in vivo.
    VL  - 10
    IS  - 3
    ER  - 

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Author Information
  • Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

  • Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

  • Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

  • Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

  • Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

  • Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

  • Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

  • College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China

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