A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra

Lamia Mohammed Abd Elhalim; Nesrin Khamis Ramadan; Mohammed Khaled Abd El Rahman; Maha Mohammed Galal

doi:doi:10.11648/j.sjac.20241202.12

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| Peer-Reviewed

A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra

Lamia Mohammed Abd Elhalim^*

, Nesrin Khamis Ramadan, Mohammed Khaled Abd El Rahman

, Maha Mohammed Galal

Published in Science Journal of Analytical Chemistry (Volume 12, Issue 2)

Received: 8 August 2024 Accepted: 2 September 2024 Published: 26 September 2024

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Abstract

In this study, the combination of pharmaceutical formulation of tetrahydrozline HCl (TZ) and antazoline HCl (AN) was determined without separating them using smart analytical UV spectrophotometric methods. While the Extended Ratio Subtraction Method (EXRSM) is used to determine AN, the Ratio Subtraction Method (RSM) is utilized to determine TZ and is linked with the ratio subtraction technique. The calibration curves for AN and TZ are linear, ranging from 3.0 to 30.0 µg/mL and 5.0 to 45.0 µg/mL, respectively. Analyzing several laboratory-prepared combinations of the two medications allowed researchers to assess the specificity of the designed methods. The selected drugs' combined dosage form was determined with success using both approaches. Validation was carried out in accordance with ICH requirements, and it was found that repeatability, accuracy, and intermediate precision were all within acceptable limits. Statistical studies showed that both methods can be competitively applied in quality control laboratories. RSM and the EXRSM are complementary to one another, as shown by the determination of AN and TZ without pre-separation. Without any prior separation, the EXRSM was able to differentiate between substances with an extended spectrum using the same characteristics. Therefore, one alternative approach to liquid chromatography techniques is the combination of EXRSM and RSM.

Published in	Science Journal of Analytical Chemistry (Volume 12, Issue 2)
DOI	10.11648/j.sjac.20241202.12
Page(s)	24-32
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antazoline, Tetrahydrozoline, Ratio Subtraction, Extended Ratio Subtraction, Spectrophotometry

References

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APA Style

Elhalim, L. M. A., Ramadan, N. K., El Rahman, M. K. A., Galal, M. M. (2024). A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra. Science Journal of Analytical Chemistry, 12(2), 24-32. https://doi.org/10.11648/j.sjac.20241202.12

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ACS Style

Elhalim, L. M. A.; Ramadan, N. K.; El Rahman, M. K. A.; Galal, M. M. A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra. Sci. J. Anal. Chem. 2024, 12(2), 24-32. doi: 10.11648/j.sjac.20241202.12

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AMA Style

Elhalim LMA, Ramadan NK, El Rahman MKA, Galal MM. A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra. Sci J Anal Chem. 2024;12(2):24-32. doi: 10.11648/j.sjac.20241202.12

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@article{10.11648/j.sjac.20241202.12,
  author = {Lamia Mohammed Abd Elhalim and Nesrin Khamis Ramadan and Mohammed Khaled Abd El Rahman and Maha Mohammed Galal},
  title = {A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra
},
  journal = {Science Journal of Analytical Chemistry},
  volume = {12},
  number = {2},
  pages = {24-32},
  doi = {10.11648/j.sjac.20241202.12},
  url = {https://doi.org/10.11648/j.sjac.20241202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20241202.12},
  abstract = {In this study, the combination of pharmaceutical formulation of tetrahydrozline HCl (TZ) and antazoline HCl (AN) was determined without separating them using smart analytical UV spectrophotometric methods. While the Extended Ratio Subtraction Method (EXRSM) is used to determine AN, the Ratio Subtraction Method (RSM) is utilized to determine TZ and is linked with the ratio subtraction technique. The calibration curves for AN and TZ are linear, ranging from 3.0 to 30.0 µg/mL and 5.0 to 45.0 µg/mL, respectively. Analyzing several laboratory-prepared combinations of the two medications allowed researchers to assess the specificity of the designed methods. The selected drugs' combined dosage form was determined with success using both approaches. Validation was carried out in accordance with ICH requirements, and it was found that repeatability, accuracy, and intermediate precision were all within acceptable limits. Statistical studies showed that both methods can be competitively applied in quality control laboratories. RSM and the EXRSM are complementary to one another, as shown by the determination of AN and TZ without pre-separation. Without any prior separation, the EXRSM was able to differentiate between substances with an extended spectrum using the same characteristics. Therefore, one alternative approach to liquid chromatography techniques is the combination of EXRSM and RSM.
},
 year = {2024}
}

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TY  - JOUR
T1  - A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra

AU  - Lamia Mohammed Abd Elhalim
AU  - Nesrin Khamis Ramadan
AU  - Mohammed Khaled Abd El Rahman
AU  - Maha Mohammed Galal
Y1  - 2024/09/26
PY  - 2024
N1  - https://doi.org/10.11648/j.sjac.20241202.12
DO  - 10.11648/j.sjac.20241202.12
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 24
EP  - 32
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20241202.12
AB  - In this study, the combination of pharmaceutical formulation of tetrahydrozline HCl (TZ) and antazoline HCl (AN) was determined without separating them using smart analytical UV spectrophotometric methods. While the Extended Ratio Subtraction Method (EXRSM) is used to determine AN, the Ratio Subtraction Method (RSM) is utilized to determine TZ and is linked with the ratio subtraction technique. The calibration curves for AN and TZ are linear, ranging from 3.0 to 30.0 µg/mL and 5.0 to 45.0 µg/mL, respectively. Analyzing several laboratory-prepared combinations of the two medications allowed researchers to assess the specificity of the designed methods. The selected drugs' combined dosage form was determined with success using both approaches. Validation was carried out in accordance with ICH requirements, and it was found that repeatability, accuracy, and intermediate precision were all within acceptable limits. Statistical studies showed that both methods can be competitively applied in quality control laboratories. RSM and the EXRSM are complementary to one another, as shown by the determination of AN and TZ without pre-separation. Without any prior separation, the EXRSM was able to differentiate between substances with an extended spectrum using the same characteristics. Therefore, one alternative approach to liquid chromatography techniques is the combination of EXRSM and RSM.

VL  - 12
IS  - 2
ER  -

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Author Information

Lamia Mohammed Abd Elhalim

Central Administration for Drug Control, Egyptian Drug Authority, Cairo, Egypt

Contact Email

http://orcid.org/0009-0002-0305-404X
Nesrin Khamis Ramadan

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt

Contact Email
Mohammed Khaled Abd El Rahman

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt

Contact Email

http://orcid.org/0000-0002-8028-5258
Maha Mohammed Galal

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt

Contact Email

http://orcid.org/0000-0001-7824-5049

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Plain Text BibTeX RIS

APA Style

Elhalim, L. M. A., Ramadan, N. K., El Rahman, M. K. A., Galal, M. M. (2024). A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra. Science Journal of Analytical Chemistry, 12(2), 24-32. https://doi.org/10.11648/j.sjac.20241202.12

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ACS Style

Elhalim, L. M. A.; Ramadan, N. K.; El Rahman, M. K. A.; Galal, M. M. A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra. Sci. J. Anal. Chem. 2024, 12(2), 24-32. doi: 10.11648/j.sjac.20241202.12

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AMA Style

Elhalim LMA, Ramadan NK, El Rahman MKA, Galal MM. A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra. Sci J Anal Chem. 2024;12(2):24-32. doi: 10.11648/j.sjac.20241202.12

Copy | Download

@article{10.11648/j.sjac.20241202.12,
  author = {Lamia Mohammed Abd Elhalim and Nesrin Khamis Ramadan and Mohammed Khaled Abd El Rahman and Maha Mohammed Galal},
  title = {A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra
},
  journal = {Science Journal of Analytical Chemistry},
  volume = {12},
  number = {2},
  pages = {24-32},
  doi = {10.11648/j.sjac.20241202.12},
  url = {https://doi.org/10.11648/j.sjac.20241202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20241202.12},
  abstract = {In this study, the combination of pharmaceutical formulation of tetrahydrozline HCl (TZ) and antazoline HCl (AN) was determined without separating them using smart analytical UV spectrophotometric methods. While the Extended Ratio Subtraction Method (EXRSM) is used to determine AN, the Ratio Subtraction Method (RSM) is utilized to determine TZ and is linked with the ratio subtraction technique. The calibration curves for AN and TZ are linear, ranging from 3.0 to 30.0 µg/mL and 5.0 to 45.0 µg/mL, respectively. Analyzing several laboratory-prepared combinations of the two medications allowed researchers to assess the specificity of the designed methods. The selected drugs' combined dosage form was determined with success using both approaches. Validation was carried out in accordance with ICH requirements, and it was found that repeatability, accuracy, and intermediate precision were all within acceptable limits. Statistical studies showed that both methods can be competitively applied in quality control laboratories. RSM and the EXRSM are complementary to one another, as shown by the determination of AN and TZ without pre-separation. Without any prior separation, the EXRSM was able to differentiate between substances with an extended spectrum using the same characteristics. Therefore, one alternative approach to liquid chromatography techniques is the combination of EXRSM and RSM.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - A Novel Analytical Method for Determining a Combination of Tetrahydrozoline and Antazoline HCl by Modulating Ratio Spectra

AU  - Lamia Mohammed Abd Elhalim
AU  - Nesrin Khamis Ramadan
AU  - Mohammed Khaled Abd El Rahman
AU  - Maha Mohammed Galal
Y1  - 2024/09/26
PY  - 2024
N1  - https://doi.org/10.11648/j.sjac.20241202.12
DO  - 10.11648/j.sjac.20241202.12
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 24
EP  - 32
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20241202.12
AB  - In this study, the combination of pharmaceutical formulation of tetrahydrozline HCl (TZ) and antazoline HCl (AN) was determined without separating them using smart analytical UV spectrophotometric methods. While the Extended Ratio Subtraction Method (EXRSM) is used to determine AN, the Ratio Subtraction Method (RSM) is utilized to determine TZ and is linked with the ratio subtraction technique. The calibration curves for AN and TZ are linear, ranging from 3.0 to 30.0 µg/mL and 5.0 to 45.0 µg/mL, respectively. Analyzing several laboratory-prepared combinations of the two medications allowed researchers to assess the specificity of the designed methods. The selected drugs' combined dosage form was determined with success using both approaches. Validation was carried out in accordance with ICH requirements, and it was found that repeatability, accuracy, and intermediate precision were all within acceptable limits. Statistical studies showed that both methods can be competitively applied in quality control laboratories. RSM and the EXRSM are complementary to one another, as shown by the determination of AN and TZ without pre-separation. Without any prior separation, the EXRSM was able to differentiate between substances with an extended spectrum using the same characteristics. Therefore, one alternative approach to liquid chromatography techniques is the combination of EXRSM and RSM.

VL  - 12
IS  - 2
ER  -

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